Multi-omics Analysis of Oral-gut Microbial Profiles

Changes in gut microbial profile measured in stool samples,Changes in oral microbial profile measured in saliva samples,Changes in oral microbial profile measured in subgingival plaque samples

Periodontitis is a chronic inflammatory disease of the tooth supporting structures induced by
 a dysbiosis in the oral and subgingival microenvironment of susceptible patients. The
 long-term swallowing of high doses of periodontal pathogenic microorganisms could induce a
 dysbiosis of the intestinal microbiota, favouring the establishment of an 'inflamed'
 microbiome in terms of composition and/or function. The present project is aimed at a better
 understanding of the etiopathogenetic correlation between periodontitis and intestinal
 dysbiosis, and aims to explore the hypothesis that periodontal treatment may influence the
 multi-omics profile on the oral-gut-systemic axis. 70 patients affected by stage III-IV
 periodontitis will be recruited, and treated by means of full-mouth scaling and root planing.
 Salivary, subgingival plaque, plasma and stool samples, together with a complete periodontal
 charting and a food diary will be collected and compared at baseline and after treatment.
 Age, gender and BMI-matched healthy individuals will be recruited as controls.