Application of Spectral Methods to Assess Gametes, Embryos, and Human Reproductive Capabilities

Embryonic model performance,Gamete model performance,Endometrial model performance,Embryonic model clinical efficiency,Endometrial model clinical efficiency

Relevance of the research topic: At present, in the world, a kind of plateau in the
 efficiency of assisted reproductive technologies has been achieved, which ensures a birth
 rate of 30% per embryo transfer. At the same time, a relatively high (15-20%) and stable rate
 of miscarriages is preserved. Until now, no effective methods for assessing the potential of
 gametes and embryos, as well as human reproductive capabilities, have been offered. In these
 conditions, to increase the rate of births after IVF, clinicians have to increase the number
 of transferred embryos at a time, however, this leads to a sharp increase in complications of
 IVF, such as multiple pregnancy. In addition, until today, the clinical effectiveness of
 assessing the potential of endometrium using gene expression determination methods has not
 been shown. Therefore, to ensure the effectiveness and safety of infertility treatment, it is
 necessary to develop methods for predicting the potential of gametes and embryos, as well as
 human reproductive capabilities. For this purpose, the investigators assume to use Raman
 spectroscopy of the environment obtained from the objects of research, as well as fluorescent
 spectroscopy of endometrium. The objects of the research are gametes (spermatozoa) and
 embryos, used culture medium, endometrium. The subject of the study is the set of factors,
 that exists in the objects of research and their ability to determine the outcomes of
 infertility treatment.