The Effect of Aerosol Formed From Tobacco Heating Systems on the Microbiome of Supragingival Dental Biofilm

diversity of bacterial species,abundance of bacterial species,caries risk

Dental caries is a multifactorial disease primarily caused by supragingival dental biofilm.
 Its progression is influenced by many environmental factors, which include smoking. Tobacco
 heating systems (THS) are a novel tobacco product whose effect on the microbiome of the
 supragingival dental biofilm has not yet been investigated.
 
 The aim of the proposed research is to determine and compare the composition of the
 microbiomes of the supragingival dental biofilm of THS smokers, cigarette smokers, and
 nonsmokers using the Next Generation Sequencing method and to assess and compare the risk of
 new caries lesion formation between the test groups using the Cariogram method.
 
 The results of this research will provide insight into changes in the microbiome of the
 supragingival dental biofilm resulting from exposure to aerosols from tobacco combustion and
 tobacco heating.