Manipulation of the Gut Microbiome by a Standardized Preoperative Diet to Prevent Colorectal Cancer Recurrence and Metastasis Following Surgery

Change of the presence of active-urokinase-type plasminogen activation (uPA) stool samples before and after diet intervention using western blot and zymography.

A single arm, prospective, single center Phase1/2 Dietary intervention trial for subjects
 diagnosed with colorectal cancer and scheduled for tumor resection more than 21 days from
 informed consent. Subjects will be provided 21 days of standardized plant-based low fat/
 high-fiber meals and complete a food diary up until lunch on the day prior to surgery. Blood
 samples will be collected at baseline and on the day of surgery. Stool samples will be
 collected throughout the dietary intervention period at specific timepoints, on the day of
 surgery, and 30 days post-surgery. Tissue from the tumor resection will be isolated for
 organoid cultures. Subjects will be followed per standard of care for up to 5 years or
 disease recurrence, whichever comes first. Subject may be treated for their cancer prior to
 surgery and/or during the follow-up period.