EUROIMMUN Anti-Collagen type VII ELISA (IgG)

Epidermolysis bullosa aquisita (EBA). The ELISA test kit provides a semiquantitative or quantitative in vitro assay for the determination of human autoantibodies of the immunoglobulin class IgG against collagen type VII. The test kit contains microtiter strips each with 8 individual break-off reagent wells coated with collagen type VII. In the first reaction step, diluted patient samples are incubated in the wells. In the case of positive samples, the specific IgG antibodies (also IgA and IgM) will bind to the corresponding antigenic site. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgG (enzyme conjugate) catalysing a colour reaction.